Journal: FEBS Open Bio
Article Title: ProNGF processing in adult rat tissues and bioactivity of NGF prodomain peptides
doi: 10.1002/2211-5463.13768
Figure Lengend Snippet: (A–C) Dot blot and western blot for commercial antibody selectivity towards proNGF and/or NGF. (A) Detection of NGF, proNGF, C1 and C6 by using the different antibodies against NGF and proNGF: Abcam ab6199, Alomone ANT‐005 and Biosensis M‐1778‐B or S‐080. (B, C) The representative western blots show the ability and selectivity of (B) Abcam anti‐NGF antibody and (C) Biosensis (M‐1778‐B) anti‐proNGF antibody to detect recombinant NGF and/or proNGF.
Article Snippet: Antibodies against proNGF (Alomone Labs, Jerusalem, Israel ANT‐005; 1 : 1000, and Biosensis, Thebarton, South Australia M‐1778‐B; 1 : 1000, S‐080; 1 : 1000), and NGF (Abcam, Cambridge, UK, ab6199, 1 : 1000) were preliminary used to evaluate their reactivity and selectivity to recognise NGF prodomain and/or its mature form by dot blot.
Techniques: Dot Blot, Western Blot, Recombinant